CLCN7 Knockout cell line (HCT 116)

CLCN7 Knockout cell line (HCT 116) Order

Catalog#

YKO-HS13737

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

CLCN7 Knockout cell line (HCT 116)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

CLCN7

Gene ID

1186

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 1 (based on transcript CLCN7-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HCT 116			STR Info (Cell bank) Cell Line:HCT 116
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	11		10	11
D13S317	10	12		10	12
D7S820	11	12		11	12
D16S539	11	13		11	13
VWA	17	22		17	17
TH01	8	9		8	9
AMEL	X	X		X	Y
TPOX	8	8		8	9
CSF1PO	7	10		7	10

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CLCN7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CLCN7 gene knockout CLCN7 Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)
More details Order

Catalog#: YC-C004

Size: 1*10^6

Instruction:

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit
More details Order

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
More details Order

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

CLCN7 Knockout cell line (U-2 OS)
inquiry More details

Organism: Human

Gene Name: CLCN7

Gene ID: 1186

Size: 1*10^6

Catalog#: YKO-H856

YKO-H856(U-2 OS)
CLCN7 Knockout cell line(Hela)
inquiry More details

Organism: Human

Gene Name: CLCN7

Gene ID: 1186

Size: 1*10^6

Catalog#: YKO-H544

YKO-H544(Hela)
CLCN7 Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: CLCN7

Gene ID: 1186

Size: 1*10^6

Catalog#: YKO-HS13736

YKO-HS13736(HEK293)

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)

Price (USD): -

Catalog#: YC-C004

Size: 1*10^6

Instruction:

inquiry More details

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

Price (USD): -;-;-

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

CLCN7 Knockout cell line (U-2 OS)

Organism: Human

Gene Name: CLCN7

Gene ID: 1186

Size: 1*10^6

Catalog#: YKO-H856

inquiry More details
CLCN7 Knockout cell line(Hela)

Organism: Human

Gene Name: CLCN7

Gene ID: 1186

Size: 1*10^6

Catalog#: YKO-H544

inquiry More details
CLCN7 Knockout cell line (HEK293)

Organism: Human

Gene Name: CLCN7

Gene ID: 1186

Size: 1*10^6

Catalog#: YKO-HS13736

inquiry More details

CLCN7 Knockout cell line (HCT 116)

Ubigene